Spinning disk confocal microscopy (SDCM) represents an alternative to LSCM. Rather than a single pinhole, a SDCM has hundreds of pinholes arranged in spirals on an opaque disk (figure 2), which rotates at high speeds. When spun, the pinholes scan …

Biological spinning disk confocal microscopy is usually conducted with high numerical aperture objectives in order to gather sufficient light from weakly fluorescent specimens that are often in excess of 10 micrometers in thickness.

Spinning disk confocal laser microscopy (SDCLM) overcomes the problems with CLSM by exploiting the multiplex principle. The sample is illuminated and detected at multiple points simultaneously. This work was originally carried out by Felgett in spectroscopy. It shows that using parallel detection delivers enhanced sensitivity.

Spinning disk confocal microscopy overcomes several weaknesses of conventional fluorescence microscopy, including removing out of focus light and using optical sectioning to construct 3-dimensional models from 2- dimensional image slices.

As an alternative, some researchers use spinning disk confocal systems, which offer much greater light throughput but somewhat diminished confocality. These systems use a rotating disk with a pattern of slits or holes installed in a microscope plane that is conjugate with the specimen.

Spinning-disk confocal microscopy is an imaging technique that combines the out-of-focus light rejection of confocal microscopy with the high sensitivity of wide-field microscopy. Because of its unique features, it is well suited to high-resolution imaging of yeast and other small cells.

Spinning Disk Confocal Microscopy - Spinning disk confocal microscopy is rapidly emerging as the technique of choice for investigation of dynamics in living cells. Modern commercial instruments and high-performance camera systems are capable of providing high acquisition speeds with acceptable contrast and minimal photobleaching at the low ...

Spinning disk confocal microscopy (SDCM) is a versatile and widely-used imaging technique in biology due to its ability to perform fast, 3D imaging of live cells.

Spinning disk confocal microscopy solves the scanning problem by using multiple pinholes. The primary means of achieving this is through arranging pinholes in a spiral pattern, etched into an opaque disk (Figure 3).

Unlike the confocal laser-scanning microscope, which takes several seconds to generate a single image (and several minutes to generate a high contrast, high resolution series of images), a spinning disc confocal microscope collects an image in a fraction of a second (and a series of images in a few seconds). This is due to the fact that the ...

Spinning disk confocal microscopy utilizes multiple pinholes or slits to project a series of 1000 or more parallel excitation light beams onto the specimen in a multiplexed pattern that is subsequently detected after fluorescence emission passes through the same pinholes or slits.

2024年7月15日 · Spinning disk confocal microscopy is a powerful imaging technique that combines high-speed acquisition with the ability to capture high-resolution, three-dimensional images of live specimens. By employing a rapidly rotating disk with multiple pinholes, this method reduces photobleaching and phototoxicity, making it ideal for long-term, live ...

2014年1月1日 · The central concept of spinning-disk confocal microscopy (SDCM), first conceived by Petrán (Petrán, Hadravsk, Egger, & Galambos, 1968), is that an array of illuminated pinholes positioned at one of the microscope's intermediate image planes will create a demagnified array of tiny excitation focal volumes at the conjugate objective lens object ...

Spinning disk confocal microscopy increases the speed of this technique by using multiple pinholes etched into an opaque disk which, when spun, scans the pinholes across the entire image.

2015年11月13日 · Spinning Disk Confocal Microscopy. Spinning disk microscopy has advanced significantly in the past decade and now represents one of the optimum solutions for both routine and high-performance live-cell imaging applications.

Rather than a single pinhole, a spinning disk confocal microscope (SDCM) has hundreds of pinholes arranged in spirals on an opaque disk, which rotates at high speeds. When spun, the pinholes scan across the specimen in rows, building up an image.

We describe an inexpensive do-it-yourself (DIY) spinning disk confocal microscope (SDCM) module based on a commercially fabricated chromium photomask that can be added on to a laser-illuminated epifluorescence microscope.

A comparative investigation of spinning disk and laser scanning confocal microscopy using signal-to-noise and photobleaching as an index of system efficiency. Included are detail discussions of instrument configuration, resolution, photobleaching sensitivity, and saturation effects.

The spinning disk confocal microscope (SDCM) is a revolutionary tool for imaging in the life sciences, observing samples ranging from single molecules to live cells, featuring high speed, 3D and multichannel acquisitions.

This setup enables high-resolution spinning disk confocal microscopy with up to four different wavelengths. The stained tissue samples were excited with lasers corresponding to their respective stains, namely 488 nm (Col-F), 561 nm (SPY555-DNA), and 638 nm (SiR-actin). They were then imaged for different magnifications and to a depth of about ...

2018年6月27日 · Spinning disk confocal microscopy is a particular configuration of confocal microscopy that is able to imaging at a faster rate. For this reason spinning disk confocal microscopy is particularly useful when imaging living samples or recording fast phenomena.