4 天之前 · This mouse page is organised to show a detailed day by day development of sytems and features with approximate timing of key events. The staging by "days" relate to in the female presence of a vaginal plug indicating that the mating occurred, see timed pregnancy. Theiler Stages divides mouse development into 26 prenatal and 2 postnatal stages. [1]

2025年1月22日 · Here, a detailed step-by-step protocol for extended ex utero mouse embryo culture is provided. The ability to grow normal mouse embryos ex utero from gastrulation to organogenesis represents a valuable tool for characterizing the effect of different experimental perturbations during embryonic development."

Theiler Stages divide mouse development into 26 prenatal and 2 postnatal stages and is based upon the publication The House Mouse: Atlas of Mouse Development by Theiler Springer-Verlag, NY (1972, 1989).

For comparison, using the human staging convention, the first two trimesters of development (“embryo”) extend from E0 to E14.5 in mice, while the last trimester (“fetus”) encompasses E15 to approximately P10 in mice.

Keywords: mouse embryo, morphogenesis, axis formation, gastrulation, epiblast, mesoderm, endoderm, cell dynamics, migration, intercalation, ingression, egression, EMT, MET, imaging Introduction A major challenge in developmental biology is the elucidation of the cellular behaviors driving morphogenesis and the molecular signals that control them.

With the advent of high-throughput 3D imaging tools that capture embryo morphology in microscopic detail, we now present the first 4D atlas staging system for mouse embryonic development using optical projection tomography and image registration methods.

2025年1月21日 · Fig. 2: Generation of embryos with parental-allele-specific DMR deletions from ESCs derived by Ha-Ha-fusion system. Fig. 3: Efficient generation of alive mice from ESCs with 8 maternal DMR ...