We performed second-dimension separation at 20 mA at 4 °C for 4 h. Proteins were transferred to PVDF membranes (Pall). For detection, we used a rabbit anti-mouse apo-AI antibody (Biodesign ...
Membrane trafficking is the process by which proteins and other macromolecules are distributed throughout the cell, and released to or internalised from the extracellular space. Membrane ...
This cover highlights the article ZBTB48 is a priming factor regulating B-cell-specific CIITA expression by Grishma Rane, Dennis Kappei and colleagues. The study identifies how ZBTB48, represented as ...
This paper examines the recruitment of the inflammasome seeding pattern recognition receptor NLRP3 to the Golgi. Previously, electrostatic interactions between the polybasic region of NLRP3 and ...
Electrostatic interactions between the NLRP3 polybasic (PB) region and negatively charged lipids on the trans-Golgi network (TGN) have been proposed to recruit NLRP3 to the TGN. In this study, we ...
We found that Vti1b was mainly localised to the Golgi apparatus and a subset of lysosomes in resting B cells ... Lysates (450.000 primary cells/25 µl) were run on 10% polyacrylamide gels and ...
Finally, the SARS-CoV-2 envelope, which, as previously mentioned, resides on ER-Golgi intermediate compartment (ERGIC) and Golgi membranes, interacts with members of bromodomain and extra-terminal ...