Nature19 小时
Detection of protein-protein interactions using the GST fusion protein pull-down technique
Discard the supernatants. GST lysis buffer consists of 20 mM Tris-Cl (pH 8.0), 200 mM NaCl, 1 mM EDTA (pH 8.0), 0.5% Nonidet P-40, 2 μg/μl aprotinin, 1 μg/μl leupeptin, 0.7 μg/ml pepstatin ...