Immune cells in the digested tissues were separated and collected by Percoll gradient centrifugation. Immune cells from PPs, MLNs, and the spleen were collected by mashing these tissues through a 100 ...

The single cell suspensions were gathered and prepared through the use of a 40-μm cell strainer for filtering (Biosharp, Hefei, China), followed by purification of the leukocyte-enriched fractions ...

Lamina propria cells were then collected and further purified via density gradient centrifugation with 40% and 70% Percoll–RPMI solution.32 LPMCs were collected from the interphase. Data were ...

This important study provides solid evidence to support the anti-tumor potential of citalopram, originally an anti-depression drug, in hepatocellular carcinoma (HCC). In addition to their previous ...

Solid VX2 tumour chips were pushed through a sterile steel strainer (mesh size 400 µm) and washed with PBS. The cell suspension was separated by gradient centrifugation using Percoll (GE Healthcare, ...

Department of Translational Medicine, Lund University, Lund SE-22100, Sweden ...