pharmacological inhibitors or gene mutants should be used to confirm data from siRNA-mediated knockdown. The final word in target validation is to reintroduce an RNAi-resistant version of the ...

One of the worries with RNAi experiments is that siRNAs might knock down or induce genes other than the intended target. For example, an siRNAs “may be perfectly complementary to a gene but also ...

While RNAi exploits the naturally occurring RNAi pathway used by cells to identify and target a specific messenger RNA for degradation, CRISPRi binds to the DNA and blocks either transcription ...

The technique: A specific short peptide aimed at fat cells can deliver a DNA sequence ... “It’s always welcome when there is a new technology to deliver specific targeting molecules, whether they’re ...