Thus, the overall efficiency of RT– and real-time PCR in detecting the housekeeping gene in a limited number of picked cells was 82% (243/297 samples). Negative samples may be mostly caused by ...

Where qualitative PCR analysis is performed ... Most of the event-specific, real-time PCR protocols validated by the Community Reference Laboratory (CRL; Ispra, Italy) have been optimized on ...

The samples were assayed for HHV-DNA (HHV1–8) by using two different polymerase chain reaction (PCR) assays, qualitative PCR (multiplex PCR) and quantitative PCR (real-time PCR). Results: In all of ...

Primerdesign (part of the Novacyt Group), a company focused on the design, manufacture, validation and supply of real-time PCR kits ... are multiplexed qualitative PCR (qPCR) assays that detect ...