2016). The produced Fshr-ZsGreen (Fshr-ZsG) knockin mice were maintained as heterozygotes, and homozygotes were used for experiments. For genotyping, genomic DNA was extracted from tail tips and ...

The pinnae of mice and rats are generally large ... ear punches can provide DNA for genotyping by polymerase chain reaction (PCR). Southern blot determination of the genotype requires a larger ...

2015). Next generation sequencing (NGS) is advantageous because there is no need to normalize the PCR for producing robust zygosity data, which could be a tool for high-throughput transgenic genotype ...

This DNA will be injected into the pronuclei of fertilized mouse eggs. Surviving embryos will be transferred to surrogate mothers and the tail tips from the pups born will be provided to the ...

Methods Wildtype (wt) and KRASG12V transgenic C57BL6/J mice received a 3 months diet enriched with PPARg-agonist (rosiglitazone, ~25 mg/kg*day, n=5-10 per group and genotype) for measurement of tumor ...

PCR products were denatured at 95°C for 1 min, rapidly cooled down to room temperature and melted from 50°C to 98°C at a melt ramp rate of 2°C/s. The fluorescence was measured in real-time with the ...