We performed second-dimension separation at 20 mA at 4 °C for 4 h. Proteins were transferred to PVDF membranes (Pall). For detection, we used a rabbit anti-mouse apo-AI antibody (Biodesign ...

Cells have extensive sets of intracellular membranes, which together compose the endomembrane system. The endomembrane system was first discovered in the late 1800s when scientist Camillo Golgi ...

She was particularly interested in the Golgi apparatus, a cluster of membrane-bound compartments that help coordinate this trafficking, and spent much of her time manipulating the organelle’s activity ...

1 Despite having been overlooked for the last century, this has turned out to be a prescient description of the interior of eukaryotic cells. In addition to membrane-encased organelles—the nucleus, ...

These results support the hypothesis that the Golgi uses a size filter to determine if a protein should be trafficked to the ...

capturing the development of nascent membrane structures within cells. They observed that although the endoplasmic reticulum (ER) exit sites and Golgi apparatus declined in numbers, they ...