We performed second-dimension separation at 20 mA at 4 °C for 4 h. Proteins were transferred to PVDF membranes (Pall). For detection, we used a rabbit anti-mouse apo-AI antibody (Biodesign ...
Cells have extensive sets of intracellular membranes, which together compose the endomembrane system. The endomembrane system was first discovered in the late 1800s when scientist Camillo Golgi ...