Gene knockout approaches completely ablate gene expression at the DNA level. 3 Researchers can accomplish this by using genome editing tools, such as zinc finger nucleases (ZFN), transcription ...

One way to understand the function of a gene is to observe a biological system that lacks that gene. But what is the best system to use? When studying human genes, researchers typically employ ...

To create a knockout mouse, scientists genetically engineer the animal by disrupting a gene of interest. This may be done by deleting portions of the gene's DNA sequence to make it nonfunctional ...

Both gene knockout and knock-in models are powerful tools widely used in biomedical research to gain important insights into ...

The Transgenic and Knockout Core can construct gene-targeting vectors for the generation of knockout, knockin and conditional knockout mice. A recombineering approach is used, and it typically takes ...

to knockout and characterize all protein-coding genes in the mouse genome. Knocking out the activity of a gene provides valuable clues about what that gene normally does. However, creating and ...

Synthego, a leading genome engineering solutions company, today announced the launch of the Gene Knockout Kit v2. This is the only product that leverages a novel ...

The Transgenic and Knockout Core can develop mice with targeted mutations in the entire mouse or in specific tissues or cell types. It normally takes about a year to generate a gene-targeted mouse.

This is a useful study that seeks to address the role of the TET family of DNA demethylation enzymes in pancreatic beta cell senescence in the context of type 2 diabetes (T2DM). Although the concepts ...