Northern blotting ... Rinse the gel with DEPC-treated H 2 0; soak for 20 min in five gel volumes of 0.01 N NaOH/3 M NaCl. 3| Place a piece of thick blotting paper on a sheet of Plexiglas or ...

Following separation, the RNA is transferred from the gel onto a blotting membrane. (Although this step is what gives the technique the name "northern blotting," the term is typically used to ...

One advantage of Northern blotting is the ease with which comparative analyses can be carried out, as researchers can run RNA samples representing different developmental time points, tissues, or ...

The Southern blot is the most common way to analyse restriction endonuclease-digested DNA to find specific sequences of interest. It uses the fact that denatured nucleic acids will bind to ...