The far-red emission of the dye facilitates multiplexing with GFP and other fluorescent probes. With the dye, cells may be analyzed by fluorescence microscopy, flow cytometry and laser-scanning ...
“Flow cytometry has been around for many years ... transfected genes carry some kind of fluorescent tag, such as a GFP molecule, the expressed protein is as close to the native configuration ...
GFP, mRFP, mCherry, tdTomato, mKate, mPlum). Applications that may benefit from access to this equipment includes: Construction and analysis of designed gene regulatory networks in bacteria.
The intuitive software can be mastered in minutes and presentations can be made by a drag and drop of plots into Microsoft Office applications. The BDFACSCelesta Flow Cytometer multicolor analyzer ...
the original CFSE being in the same channel as GFP. If violet laser diodes are not available, a Red HeNe diode can be used to measure cell proliferation by the use of Cell Trace Far Red DDAO-SE. Cell ...
This has advanced the use of flow cytometry in an increasing number of ... special applications such as side population assay, basic GFP sorting or optimized for multiple fluorescent protein ...