For fluorescence, this microscope uses an Excelitas Excite 100-watt metal halide lamp, Chroma optical filters, and is capable of imaging DAPI/Hoechst, EGFP, TxRed/mCherry/Alexa 568, Alexa 647 and ...
It surpasses the diffraction limit of conventional light microscopy by utilizing the properties of fluorescent proteins or dyes that can be photoactivated or photoswitched. PALM enables the ...