The basic scheme here is known as confocal laser scanning fluorescence microscopy, where a laser ... to read pits as small as 150 nm on a Blu-Ray DVD, are well-suited for resolving similarly ...

Out-of-focus light (red and blue dash lines in diagram) is eliminated because it follows a different light path and thus does not go through the pinhole. In laser-scanning confocal microscopy, a small ...

Thus, as with image- scanning microscopy technologies, the Airyscan detection concept can be leveraged with multiphoton excitation to increase the imaging depth beyond that of traditional confocal ...